Figure 1

MIF induces neutrophil accumulation into the alveolar space. Mice were euthanized at 3, 6 or 20 hours post instillation of 1.0 μg MIF or 3 hours (saline controls). Lungs were lavaged three times with normal saline. A. The number of neutrophil in the bronchoalveolar lavage (BAL) fluid was counted by hemocytometer. Bars indicate mean ± SD (n = 6/group). *P < 0.05 compared with normal saline control. B. (i), (iv) show the cell population in BAL fluid with HEMA3 stain. (i)-(iii) shows representative samples from a control mouse, and (iv)-(vi) shows samples from 6 hours after MIF instilled mice. Lung tissue stained with H-E stain in (ii), (v) and naphthol AS-D chloroacetate esterase stain (neutrophil granules were stained with bright red color, point with arrows) in (iii), (vi).