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Figure 1 | Respiratory Research

Figure 1

From: Extracellular Hsp72, an endogenous DAMP, is released by virally infected airway epithelial cells and activates neutrophils via Toll-like receptor (TLR)-4

Figure 1

RSV infection activates the stress response in human bronchial epithelial cells. A. 16HBE14o- cells were infected with RSV at 1 – 10 MOI and intracellular proteins were harvested for Western immunoblot at 6 h. RSV infection resulted in a dose-dependent increase in intracellular Hsp72 expression. This experiment is representative of two separate experiments. B. 16HBE14o- cells were infected with RSV at 1 – 10 MOI for 18 h and supernatants were isolated. Hsp72 levels in the supernatant were assayed by ELISA. Data represent means ± SEM for 3 separate experiments and differences pinpointed by ANOVA. RSV infection resulted in a significant increase in extracellular Hsp72 release (* p = 0.002 and **p = 0.006 compared to uninfected). C. Quantitative real time PCR was expressed as Hsp72 normalized to SDHA and expressed as fold increase over control is shown (compared to uninfected *p = 0.044, **p = 0.003, n = 3).

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Respiratory Research

ISSN: 1465-993X

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