Figure 8
PDGF-AA suppresses α-smooth muscle actin gene expression in sub-confluent mouse lung fibroblasts. The neonatal mouse lung fibroblast cell line, Mlg, was stimulated with PDGF-AA (AA) for 24 h and levels of α-SMA mRNA for (A) increasing concentrations of PDGF-AA in sub-confluent cells (4.5 × 105 cells at the time AA was added), or (B) sub-confluent versus confluent cells (4.7 × 106 cells) was measured using real-time quantitative RT-PCR, with beta-2-microglobulin as the control "housekeeping" gene. For each reverse-transcribed RNA, 3 triplicate amplifications were performed. The error bars are 1 SD, columns are means of independent experiments. # = p < 0.05 compared to vehicle (veh).