Figure 7
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Immunostaining of SP-A. Fluorescence microscopic pictures of sections immunostained with SP-A antibody (green) and nuclear DAPI staining (blue). For fetal lung tissue (22 weeks old) (magnification 200×) (A) and hESC cultured with the ALI differentiation protocol at day 20 (VUB03_DM1 (B (magnification 400×) Pictures of sections in a perpendicular direction are shown on three different cell lines: VUB07 (C), VUB03_DM1 (D) and VUB04_CF (E).