Figure 1
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Relative quantification of CC16 and NKX2.1 mRNA levels in four different hESC lines at day 5, 10, 15 and 20 of ALI culture. Relative quantification of CC16 (A) and NKX2.1 (B) mRNA levels in samples from human embryonic stem cells (hESC) cultured on porous membranes according to the ALI differentiation protocol. Samples were collected on days 5, 10, 15, and 20 of ALI culture. A peak expression is seen at day 10, with a 1000 and 10000- fold expression, followed by a decrease further over time. The NO ALI control samples were considered as reference. (Note that the y-axis is on a logarithmic scale). Two endogenous controls were used (GAPDH and Ubiquitin C). These experiments were repeated at least three times with VUB03_DM1, VUB04_CF, VUB07 and VUB14 hESC lines and data are presented here as means.