Molecular imaging of pulmonary diseases

Imaging holds an important role in the diagnosis of lung diseases. Along with clinical tests, noninvasive imaging techniques provide complementary and valuable information that enables a complete differential diagnosis. Various novel molecular imaging tools are currently under investigation aimed toward achieving a better understanding of lung disease physiopathology as well as early detection and accurate diagnosis leading to targeted treatment. Recent research on molecular imaging methods that may permit differentiation of the cellular and molecular components of pulmonary disease and monitoring of immune activation are detailed in this review. The application of molecular imaging to lung disease is currently in its early stage, especially compared to other organs or tissues, but future studies will undoubtedly reveal useful pulmonary imaging probes and imaging modalities.


Background
Imaging holds an important complementary role in the diagnosis of lung diseases. Along with clinical tests, imaging techniques provide valuable information that enables a complete differential diagnosis. Often, lung disease symptoms are similar and begin to be discriminative only at a late stage when prognosis of the ultimately named pathology is already less favorable. Currently, chest X-ray, computed tomography (CT) and magnetic resonance imaging (MRI) are conventional imaging modalities first prescribed after patient examination. With high spatial resolution, these modalities provide anatomical and structural information of the lungs, but in order to differentiate between lung diseases with overlapping pathophysiology or to enable diagnosis at a very early stage, a more accurate approach is necessary. To this extent, the emerging field of molecular imaging is of great relevance. Molecular imaging is a field at the intersection of molecular biology and conventional medical imaging [1]. Unconventional imaging modalities such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) exhibit high sensitivity and resolution to provide information at a molecular level. Table 1 lists the general characteristics as well as the costs of conventional and unconventional imaging modalities [2]. The combination of structural and molecular information is of great necessity to enable individualization of pulmonary pathologies and open novel avenues for advancing personalized medicine in the field of therapeutic diagnostics (theranostics), a companion diagnostic system where the molecular targeting probe is used to carry a dye-or gamma photon-based isotope to image target expression and also to carry a therapeutic compound to the very same target. In this review, we describe principal pulmonary diseases, clinical diagnosis issues, and molecular imaging tools currently available or being studied.

Lung cancer
Lung cancer is the leading cause of cancer-related death in men worldwide, and the second leading cause in women [3]. In the United States, lung cancer is the leading cause of cancer death in both men and women [3]. It is estimated that greater than 25% of cancer-related deaths in the United States in 2017 will be caused by lung cancer, and that it will kill more people than breast, colon, and prostate cancer combined [4]. Lung cancer has three main histologic categories: non-small cell lung cancer (NSCLC), small-cell lung cancer (SCLC), and neuroendocrine tumors (NET) [5]. NSCLC is most common, accounting for 85-90% of all lung cancers, and is made up of squamous cell carcinoma, adenocarcinoma, and large cell carcinoma. The main risk factor for the development of lung cancer is smoking. Other risk factors include exposure to air pollution, second-hand smoke, radiation (either radon decay or from medical Xrays), and asbestos [5]. Independent of type, early and accurate diagnosis of lung cancer is important to increase the likelihood of survival. Chest radiographs are the first line of conventional imaging used to detect abnormal areas in the lung but are limited in their capacity to detect cancer due to low-resolution and the twodimensional nature of the image. Low-dose spiral CT is therefore the gold standard for lung cancer diagnosis and staging [5]. The National Lung Screening Trial found that patients at high-risk for lung cancer randomized to yearly CT scans had a 20% reduction in lung cancer mortality compared with patients randomized to yearly chest radiographs [6]. However, low-dose CT does have limitations, including a higher dose of radiation, more frequent detection of questionable lesions leading to potentially unnecessary biopsies or surgical intervention, and inability to detect chest wall invasion [7,8]. Due to the limitations of low-dose CT and the fact that lung nodules are often found incidentally, patients with lung cancer would benefit from improved imaging modalities that can provide early detection, diagnosis, and appropriate staging.
Solitary pulmonary nodule (SPN) is the name given to a small isolated dense region observed on chest X-ray and CT scans. SPNs are sources of preoccupation since they can be of benign or malignant causes such as inflammation, infection or lung cancer [9]. PET/CT and its high detection sensitivity associated with molecularly targeted probes can permit further distinction. In this regard, [ [12]. Artificial amino acid tracers such as O-[ 18 F]-fluoromethyl-L-tyrosine (FMT) [13], O-[ 18 F]-fluoropropyl-L-tyrosine (FPT), and O-(2-[ 18 F]-fluoroethyl)-L-tyrosine (FET), have also been evaluated as amino acid metabolism and protein synthesis is enhanced in tumors [14].
Angiogenesis is a phenomenon of great interest in oncology. The more a tumor is vascularized, the higher is the risk of metastasis [15][16][17]. Expression of vascular endothelial growth factor receptor type 2 (VEGFR-2) in tumors has been proportionally correlated to poor patient prognosis. Luo et al. developed a PET [ 64 Cu]-labeled monoclonal antibody targeted to VEGFR-2 [18]. In this study, the investigators were able to successfully discriminate high versus low VEGFR-2 expression levels in a mouse model bearing human NSCLC tumors using [ 64 Cu]-NOTA-ramucirumab. Such capability would allow effective monitoring of the efficacy of therapeutic treatments with angiogenesis inhibitors in lung cancer.
Hypoxia has been observed and characterized in many tumors. Hypoxic cells are phenotypically different compared Cost per scan may vary depending on the injected dose price. Adapted from [2] to the non-hypoxic cells that might have been originally used to validate the efficacy of the treatment. Hypoxia is one of the main players that will determine therapy efficacy. Nitroimidazole derivatives have been used in the development of molecular imaging tracers. [ 18 F]fluoromisonidazole-1-(2-nitroimidazolyl)-2-hydroxy-3fluoropropane (FMISO) and [ 18 F]-fluoroazomycin-arabinofuranoside (FAZA) showed strong correlation with measurements of tumor oxygenation [19]. However, FAZA exhibited a better signal-to-background ratio than FMISO due to FAZA hydrophilicity inducing fast clearance in preclinical studies [20]. Clinically, little impact has been made by hypoxia imaging tracers in lung cancer mostly due to the lack of understanding of the mechanistic actions of these imaging agents [19]. Matrix metalloproteinases (MMPs) are key players in cancer progression and are known to be overexpressed in lung tumor tissue [21]. Fluorescent bioactivable probes such as MMPSense 680 showed potential to delineate tumor mass in mutant K-ras mice [22]. This study demonstrated the ability of MMPSense 680 to discriminate normal tissue from adenoma or adenocarcinoma via fluorescent molecular tomography. Unfortunately, due to the lack of tissue penetration depth, the translation of fluorescent probes to a non-invasive clinical application is not yet feasible. However, intraoperative molecular imaging using such probes can be of tremendous help for surgeons as it facilitates tumor delineation, thus confirming that complete removal of tumor residue during surgical resection is possible [23].
In lung cancer as in other cancers, conventional imaging provides evidence for the existence of small tissue abnormalities or lesions to help guide decision making regarding whether to further investigate for malignancy. Among the various imaging modalities used in nuclear medicine, PET is the closest modality to CT imaging in terms of size limits of detection. The primary clinical role of PET imaging is the detection of anomalous regions of [ 18 F]-FDG uptake, which are often indicative of malignant lesions. The limits of detectability of tumors depend on many parameters including location of the tumor and imaging isotope. Modern clinical PET scanners have a resolution limit of 4 mm, corresponding to the detection of tumors with a volume of 0.2 ml (7 mm diameter) in 5:1 tumor-to-background ratio; whereas the minimum lesion size that can be measured with CT is about 3 mm [24]. Significant research efforts are underway to develop new detector materials, improved camera design, and new reconstructive algorithms in order to improve the limits of tumor detectability.

Acute respiratory distress syndrome (ARDS)
ARDS is an acute, progressive inflammatory lung disease that affects 10% of all intensive care unit (ICU) patients worldwide [25,26]. The pathophysiology of ARDS includes lung inflammation leading to increased permeability of the pulmonary vasculature, accumulation of pulmonary edema, increased lung weight, and a reduction in lung surface area participating in gas exchange [27]. ARDS is diagnosed clinically using the Berlin definition, which includes timing of onset, appearance of chest imaging, origin of edema, and oxygenation capacity. Timing of onset for ARDS is within 1 week of new or worsening respiratory symptoms or of a known respiratory insult. Common risk factors for the development of ARDS include trauma caused by aspiration, burns, blood transfusion and noncardiogenic shock. However, most ARDS cases are mainly caused by pneumonia and sepsis [25,28].
Many different viral, bacterial, and fungal organisms cause respiratory infections [29]. Collectively, these infections are one of the most prevalent worldwide public health problems [30]. Although many community-acquired respiratory infections are short-lived and are successfully managed as an outpatient with little to no treatment, respiratory infections cause more morbidity and mortality in the United States than any other infection [29]. The mortality rate for respiratory infections has remained rather stagnant for the past 50 years [31]. Patients who acquire a respiratory infection and are elderly, immunocompromised, or have multiple comorbidities or baseline lung dysfunction, may require inpatient hospitalization, ICU admission, mechanical ventilation, and broad-spectrum antibiotics. Respiratory infections are diagnosed through a combination of a thorough history and physical exam, clinical indicators (leukocytosis, presence of microorganisms in sputum culture), and imaging.
Common imaging modalities useful in the diagnosis of respiratory infections are chest X-ray and CT [32]. Both chest X-rays and CT scans provide nonspecific, supporting evidence for the presence of a respiratory infection, but they do not allow for identification of the etiology of a specific infection or the involved immune cell types. This lack of clinically available imaging techniques that could help identify the specifics of an infection impacts the clinician's ability to provide timely, targeted therapeutic interventions. The use of molecular imaging may allow for earlier identification of patients who are progressing towards ARDS. Additionally, the ability to image respiratory infections using molecular markers may provide important information regarding the likely etiology of the infection (i.e. bacterial versus viral). This improvement in diagnosis may allow for earlier, targeted antibiotic treatment and protection from overtreatment when it is not likely to be beneficial.
Ex vivo administration of [ 111 In]-labeled leukocytes are useful tools to detect infection [33]. In a cohort of 145 patients, administration of [ 111 In]-WBCs showed a 94% sensitivity and 64% specificity for detecting pulmonary infections [34]. In addition, a 99% negative predictive value has been observed in 137 immuno-deficient patients without focal pulmonary activity [35]. The detection of bacterial load by targeting specific prokaryotic signatures is an alternative method that can be achieved using molecularly targeted probes. For instance, a radiolabeled nucleoside analog that is an exclusive substrate of bacterial thymidine kinase (TK), has been studied for this purpose. 2′-fluoro-2′-deoxy-1β-D-arabinofuranosyl-5-[ 124/5 I]iodouracil ([ 124/5 I]-FIAU) was injected in a mouse model of lipopolysaccharide (LPS)-induced inflammation as well as an E. coli RS218 TK-positive infected mouse lung model (Fig. 1) [36]. The investigators were able to discriminate the inflammation state from infection state in the mice, reporting an [ 124/5 I]-FIAU uptake 4 times greater (1.69 ± 0.40 vs. 7.14 ± 1.09 %ID/g, respectively). In vivo SPECT/CT images confirmed these results. However, the use of [ 125 I] is usually not recommended for SPECT imaging purposes as the emitting energy is very low. Switching to PET would improve the quality of the images as well as quantitative analysis. Unfortunately, [ 125 I]-FIAU PET has only been tested in humans for infection condition that is not lung related and did not show suitable results [37].
Bacterial ribosomal RNAs have also been studied as a bacterial marker. Chen   [ 11 C]-donepezil, a marker of acetylcholinesterase [39]. Using a murine model of Staphylococcus aureus infection as well as pigs displaying post-operative abscesses, the authors demonstrated a different accumulation kinetics compared to [ 18 F]-FDG, suggesting that these two radiotracers image different aspects of immune response. Human data showed higher [ 11 C]-donepezil activity at sites of chronic inflammation when compared to [ 18 F]-FDG.
Radiolabeled antibiotics have been thought to be promising as they would be targeting specific bacteria items. Many antibiotics have been tested to this purpose [40]. Among them, ciprofloxacin, a member of the fluoroquinolone family, binds to gram-negative and gram-positive bacterial DNA gyrase. When radiolabeled with [ 99m Tc], ciprofloxacin penetrates mammalian cells and tissues but with low affinity and quick wash out. However, after a prolonged time, [ 99m Tc]-ciprofloxacin is retained at sites of infection allowing a target-tobackground ratio favorable for imaging. High sensitivity (>90%) has been found in tuberculosis and other conditions reported from a large multicenter study [41]. However, a PET version of ciprofloxacin radiolabeled with [ 18 F] showed rapid wash-in, wash-out and non-specific binding in the infected area, which depreciate the suitability of this radiotracer for infection sites [42]. Another fluoroquinolone-complex named norfloxacin dithiocarbamate (NFXDTC) has been well-characterized [43]. Zhang et al. radiolabeled NFXDTC with [ 99m Tc] and showed a 3.43±0.53% injected dose per gram (%ID/g) of infected area compared to 1.76±0.20%ID/g obtained with [ 99m Tc]-ciprofloxacin. However, lung and liver uptake are very elevated which prevent its use in related conditions. As many radiolabeled antibiotics have been or still are under investigation, none has yet demonstrated significant capacity to differentiate sterile inflammation from infection [40].

Chronic lung disease
Chronic respiratory diseases, including chronic obstructive pulmonary disease (COPD) and asthma, cause significant morbidity and mortality worldwide, accounting for 4 million deaths annually [44,45]. COPD, which includes emphysema and chronic bronchitis, can be suspected based on the presence of dyspnea, chronic cough, chronic sputum production, recurrent respiratory infections, and are associated with known risk factors such as exposure to tobacco smoke and occupational vapors [46,47]. The gold standard for diagnosing COPD is the pulmonary function test (PFT), which uses spirometry to measure lung volumes. A post-bronchodilator ratio of the fraction of expired volume in one second to the forced vital capacity (FEV1/FVC) of less than 0.70 is indicative of persistent airflow limitation [46]. The mainstay of treatment for COPD is bronchodilation, using either short-or long-acting β2-adrenergic agonists or anticholinergic agents, depending on the severity of symptoms [46].
The role for conventional imaging modalities (chest xrays and CT scans) in the diagnosis of COPD and other chronic lung diseases is to identify structural abnormalities (air trapping, airway septal thickening) and to rule out concomitant diseases that may be responsible for the patient's respiratory symptoms. Unfortunately, these conventional modalities are limited in their ability to diagnose chronic lung diseases. Chest x-rays, while inexpensive and associated with low radiation exposure, are two-dimensional and have low resolution (Table 1). CT scans are significantly more valuable due to the increased spatial resolution and improved depth of penetration; however, they are expensive and require higher radiation doses (Table 1). Additionally, research has shown that the visual evaluation of CT scans has low reproducibility [48]. However, the use of densitometric evaluation improves the utility of CT scans in diagnosing chronic lung disease [49].
Asthma is another common chronic respiratory disease that can cause airflow obstruction and symptoms similar to COPD but differs in that asthma is reversible with proper treatment. Asthma is characterized by intermittent dyspnea, cough, and wheezing. Intermittent asthma is managed with short-acting β2-adrenergic agonists such as albuterol, while persistent asthma is treated with inhaled corticosteroids [50]. PFTs are used during the diagnostic workup of asthma, however results are often normal considering that asthma symptoms occur intermittently. Additionally, the use of bronchodilators during PFTs will demonstrate the reversible nature of the airway obstruction associated with asthma, which differs from the irreversible nature of COPD. Chest xrays and CT scans have little role in the diagnosis of asthma and will usually not demonstrate any structural abnormalities. Asthma affects 7.5% of the population in the United States, with onset usually in childhood or as a young adult [50]. A family history of asthma, exposure to environmental toxins such as tobacco smoke, and low socioeconomic status are risk factors for the development of asthma [50]. The hallmark of airway inflammation in patients with asthma is allergen-specific IgE-and eosinophil-mediated neutrophil activation. Additional physiologic changes include mast cell degranulation and infiltration of T-helper lymphocytes, and is associated with elevated levels of IL-4, IL-5, and IL-13 [51][52][53][54].
Currently, planar lung scintigraphy is usually prescribed to visualize distribution of ventilation and perfusion in the lungs of patients with pulmonary embolism but also in cases of emphysema patients that undergo lung volume reduction surgery. However, use of 3dimensional ventilation/perfusion (v/q) SPECT imaging methods have increased the diagnostic accuracy and expanded the application range to various pulmonary diseases. Technegas, 99m Tc-labelled carbon particles, has been tested as an aerosol in COPD patients [55]. With a moderate to strong correlation with spirometric lung function, Technegas has potential to provide valuable information for the diagnosis of COPD. However, more studies that link v/q SPECT to lung function need to be performed to fully validate the method.
Considering that conventional imaging modalities play a limited role in the diagnosis and management of chronic respiratory diseases, there exists a significant opportunity to develop cell type-specific molecular imaging strategies than can improve diagnosis, provide monitoring of treatment responsiveness, and limit invasive and often traumatic tissue biopsies. In addition, molecular imaging could have a role in quantifying with accuracy lung involvement in COPD, asthma and other chronic pulmonary diseases. The involvement of immune cells in the development of chronic lung diseases is an important topic, which was addressed in a study by Jones et al. that assessed neutrophilic infiltration and presence of macrophages in a small group of COPD and asthmatic patients [56]. Using PET imaging associated with [ 18 F]-FDG to assess metabolism and [ 11 C]-PK11195 as a marker of macrophages, the investigators found a 2.7and 2.5-fold increase in [ 18 F]-FDG uptake in COPD patients versus, respectively, asthmatic and healthy patients whereas [ 11 C]-PK11195 tissue-to-plasma ratios were equally higher in asthma and COPD conditions compared to normal patients. The difference in [ 18 F]-FDG uptake between COPD and asthma might come from a different state of neutrophil activation between the two conditions. [ 18 F]-FDG avidity to neutrophil activation has also been demonstrated and quantified by PET imaging methods (Fig. 2) [57,58].
MMPs are known to play an important role in lung inflammation and remodeling. Using a transgenic mouse model that exclusively expresses IL-13 in lungs and an RP805 precursor [ 99m Tc]-labeled macrocyclic MMPtargeted tracer, Golestani et al. obtained a significant 2.5-fold increase in tracer uptake in these mice compared to wild-type animals via SPECT/CT imaging (Fig. 3) [59]. RP805 exhibited good correlation with CD68 expression (r = 0.70, P < 0.01). Other radiotracers such as [ 68 Ga]-DOTANOC and [ 111 In]-DTPA-A20FMDV2 may be useful for detection of fibroblast activity and AvB6-integrin expression in pulmonary fibrosis, respectively [60,61].

Lung transplant Primary graft dysfunction (ischemia-reperfusion injury)
Lung transplantation is the standard of care for patients with end-stage pulmonary disease who have failed to respond to medical management [62]. Unfortunately, outcomes after lung transplantation are worse than outcomes for all other solid organs, with a 5-year mortality rate of 50% [62,63]. Many different efforts are being explored that may improve the frequency and success rate of lung transplantations, including the use of ex vivo lung perfusion and increased utilization of organs donated after circulatory death (DCD) [64,65]. In the acute postoperative period, ischemia-reperfusion injury (IRI) leading to primary graft dysfunction (PGD) is the major cause of early morbidity and mortality [66]. IRI is characterized by endothelial cell disruption, activation of alveolar macrophages and invariant natural killer T cells, neutrophil infiltration, and the release of pro-inflammatory cytokines and reactive oxygen species [67][68][69][70]. A clinical diagnosis of severe PGD (ratio of partial pressure of arterial oxygen to fraction of inspired oxygen [PaO 2 /FiO 2 ] < 200 mmHg) is diagnosed in approximately 30% of patients within the first 72 h following lung transplantation. In addition to affecting short-term outcomes, PGD is also a significant risk factor for the development of bronchiolitis obliterans (also termed chronic lung allograft dysfunction), the leading cause of mortality 1 year following lung transplantation [71][72][73].
Currently, diagnosing PGD is limited to the use of conventional imaging methods such as chest radiography and CT scans, along with functional measurements of oxygenation capacity and compliance. The inability to rapidly and accurately diagnose PGD early after lung transplantation via non-invasive techniques restricts expanding the donor pool to include the use of marginal donor lungs (i.e. DCD lungs), which are associated with higher rates of IRI and PGD. Developing molecular imaging modalities to specifically target active inflammation and innate immune cell activation may allow for earlier diagnosis and earlier, targeted intervention.
Unfortunately, use of molecular imaging remains unexploited to address this clinical need. [ 18 F]-FDG might be used as a general indication of inflammation in the lung, but knowing the activation status of specific innate immune cell populations at various stages would not only help to understand the physiopathology, but would also help to counteract any harmful effects caused by their activation or infiltration at the site of inflammation. Immune cell-specific molecular imaging tools are numerous. Although most of these have not been tested directly in pulmonary diseases, many have demonstrated preclinical promise in other inflammatory indications. For example, [ 99m Tc]-labeled-C2A domain of synaptotagmin I has been evaluated as a marker of necrosis and apoptosis in a rat model of myocardial IRI [74]. Dynamic SPECT images showed a clean hot spot in the wall of the left ventricle at 10 min post-injection that was retained over 2 h. The signal-to-vial myocardium and signal-to-lung ratios were 4.5 ± 0.2 and 8.2 ± 0.6, respectively. Myocardial apoptosis has also been studied in a rat IRI model using a PET imaging agent, [ 18 F]WC-4-116, that targets caspase 3 activation [75]. The signal via myocardial autoradiography as well as via in vivo PET images had a 2-fold activity increase compared to myocardial area considered not at risk.
Recently, Liu et al. reported the use of a CCR2-binding peptide (64Cu-DOTA-ECL1i) for PET imaging of lung IRI in a murine lung transplant model [76]. This study, which targeted CCR2+ cells (monocytes), introduced the powerful potential of cell-specific molecular imaging techniques for noninvasive diagnosis of lung IRI. Our laboratory has begun to investigate molecular imaging methods to provide a more rapid and accurate diagnosis of lung IRI. As such, we have recently utilized [ 99m Tc]-cFLFLF, a PEGylated peptide ligand that binds formyl peptide receptor 1 (FPR1) on activated neutrophils, to perform SPECT/CT imaging in a murine model of left lung IRI [77]. Here, lung maximum intensity projections (MIP) were correlated with lung function after varying times of reperfusion. Left-to-right lung ratios of MIP Fig. 3 In vivo small-animal SPECT/CT imaging of MMP activation. a-c Examples of coronal (left) and transversal (right) views of fused small-animal SPECT/CT images of WT mice injected with RP805 (a) and CC10-IL-13 Tg mice injected with RP805 (b) or its control, amide analog tracer (c). d Small-animal SPECT-derived quantification tracer uptake in lungs. N = 5, 6, 13, and 5, respectively, for WT mice injected with RP805 and CC10-IL-13 Tg mice injected with RP805 or amide, control analog. *P, 0.01. **P, 0.001. cpv 5 counts per voxel; ID 5 injected dose. (This research was originally published in JNM [59]. © by the Society of Nuclear Medicine and Molecular Imaging, Inc.) and probe uptake were 3.8-and 2.8-fold higher, respectively, compared with sham animals (Fig. 4), which correlated with resolution of injury over time as measured by lung function and histologic presence of neutrophils. These results suggest that [ 99m Tc]-cFLFLF SPECT imaging may be a means to accurately provide quantifiable, noninvasive diagnosis of lung IRI as well as enable monitoring of the resolution of this injury over time.

Graft rejection
In addition to IRI and PGD, allograft rejection is another serious complication following lung transplantation that contributes to poor outcomes. Cell-mediated (recognition of foreign human leukocyte antigens by recipient T lymphocytes) and antibody-mediated (binding of recipient antibodies to donor cell antigens) rejection can occur both in the short-and long-term following lung transplantation [78]. Diagnosing allograft rejection can be difficult due to variations in clinical presentation, with some patients being completely asymptomatic. Transbronchial biopsies are therefore required and are the gold standard for diagnosing rejection [78]. Given the inherent risks associated with transbronchial biopsies (bleeding, infection, pneumothorax), a noninvasive molecular imaging strategy using cell type-or antibodyspecific labeling could provide a significant advancement in the diagnosis and surveillance of allograft rejection.
As previously mentioned for PGD, molecular imaging for acute or chronic lung rejection has not been widely studied. However, methods used to visualize cellular and molecular components of rejection such as cell membrane disruption, apoptosis and monitoring of immune cell activation in heart rejection may be applied to the lung [79]. In addition, a study by Chen et al. demonstrated that [ 18 F]-FDG uptake was significantly increased in a mouse model of orthotopic lung transplantation and rejection [80]. These authors demonstrated that [ 18 F]-FDG uptake occurred largely in CD8(+) T cells and was markedly attenuated following T cell depletion therapy.

Conclusions
Despite efforts in the use [ 18 F]-FDG PET imaging to detect inflammatory sites in the lung, its non-specific characteristic prevents any differential diagnosis of pulmonary disease or injury. When it comes to accurate diagnosis, staging, or post-treatment monitoring of disease, the use of molecular imaging would be a valuable tool to complement conventional imaging modalities. One of the biggest challenges ahead will be utilizing the optimal combination of imaging modality and molecular probe(s) in order to obtain an accurate and differential diagnosis. It is likely that accurate diagnoses of some pathologies will require the use of multiple molecular probes, especially in cases that involve numerous cell populations and complex or overlapping cell signaling pathways. For example, use of a neutrophil-specific probe, by itself, would not differentiate between various neutrophil-mediated inflammatory conditions in the lung such as infection, ischemia-reperfusion injury, antibody-mediated rejection, or ARDS. Although PET would provide high-resolution images, if needed, PET imaging is limited to a single radionuclide-labeled probe. However, combining imaging data with other clinical parameters will always greatly facilitate a more accurate diagnosis. One advantage of SPECT imaging is that multiple molecular probes (linked to different radionuclides) could be used to simultaneously monitor the status of various cell populations (or signaling pathways) in the lung to discriminate between overlapping pathologies such as infection, inflammation or rejection. However, a disadvantage of SPECT is its lower resolution versus PET.
Current molecular imaging methods under development, which allow visualization of specific cellular and molecular components of pulmonary disease including immune cell activation, show promise. However, thus far, the application of molecular imaging in pulmonary disease remains an under-explored field, and further studies are warranted in order to explore and develop novel cellspecific molecular probes and non-invasive imaging methods to provide differential diagnosis of lung disease. These future studies will undoubtedly reveal useful pulmonary imaging probes and imaging modalities.