Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung

Background Bronchus-associated lymphoid tissue (BALT) is the secondary lymphoid tissue in bronchial mucosa and is involved in the development of bronchopulmonary immune responses. Although migration of lymphocytes from blood vessels into secondary lymphoid tissues is critical for the development of appropriate adaptive immunity, the endothelia and lymphocyte adhesion molecules that recruit specific subsets of lymphocytes into human BALT are not known. The aim of this study was to determine which adhesion molecules are expressed on lymphocytes and high endothelial venules (HEVs) in human BALT. Methods We immunostained frozen sections of BALT from lobectomy specimens from 17 patients with lung carcinoma with a panel of monoclonal antibodies to endothelia and lymphocyte adhesion molecules. Results Sections of BALT showed B cell follicles surrounded by T cells. Most BALT CD4+ T cells had a CD45RO+ memory phenotype. Almost all BALT B cells expressed α4 integrin and L-selectin. In contrast, 43% of BALT T cells expressed α4 integrin and 20% of BALT T cells expressed L-selectin. Almost all BALT lymphocytes expressed LFA-1. HEVs, which support the migration of lymphocytes from the bloodstream into secondary lymphoid tissues, were prominent in BALT. All HEVs expressed peripheral node addressin, most HEVs expressed vascular cell adhesion molecule-1, and no HEVs expressed mucosal addressin cell adhesion molecule-1. Conclusion Human BALT expresses endothelia and lymphocyte adhesion molecules that may be important in recruiting naive and memory/effector lymphocytes to BALT during protective and pathologic bronchopulmonary immune responses.


Background
The lower respiratory tract is continually exposed to a wide variety of airborne antigens and microorganisms. The generation of rapid, specific adaptive immune responses to inhaled antigens and pathogens is critical for survival. Unfortunately, in some inflammatory and infectious diseases, such as asthma and tuberculosis, these immune responses can damage the lungs and bronchi. Thus, the bronchopulmonary adaptive immune system is involved in the maintenance of health and the development of diseases of the lower respiratory tract.
In the initiation stage of an adaptive immune response, naive T cells migrate through blood vessel high endothelia venules (HEVs) into secondary lymphoid tissues, where they are stimulated by antigen-bearing dendritic cells. This leads to the generation of antigen-specific effector and memory T cells and B cells, which are released from the secondary lymphoid tissue into the bloodstream. In the effector stage of the adaptive immune response, some of the memory T cells and B cells migrate from blood vessels into nonlymphoid tissue(s) that contain the cognate antigens or pathogens [1][2][3][4]. The lower respiratory tract has two distinct types of secondary lymphoid tissues: bronchus-associated lymphoid tissue (BALT) and lymph nodes (LNs) [5][6][7]. The presence of BALT in adult mammals depends on species, antigen stimulation and age [6][7][8]. BALT is found in normal lungs of most healthy adult rabbits, rats, guinea pigs and old adult mice [5,6,[9][10][11][12][13]. In contrast, the presence and frequency of BALT in normal lungs of healthy adult humans is controversial [5,[14][15][16][17][18][19][20].
BALT is the site of initial presentation of inhaled antigens, which are transported from the bronchial lumen by specialized cells (likely M cells and/or dendritic cells) in the epithelia over the BALT, to naive T cells, which enter the BALT through blood vessel HEVs [21][22][23][24][25][26]. Intravascular lymphocytes must bind to the endothelial surface of HEVs in BALT and other secondary lymphoid tissues, prior to migrating through the vessel wall into the tissue [1,9,27]. Thus, the adhesion molecules expressed on the surface of the HEVs help determine which subsets of lymphocytes can migrate into the tissue. There are three distinct, tissueselective combinations of adhesion molecules on HEVs in secondary lymphoid tissues. Most HEVs in peripheral LNs of mice and humans express peripheral node addressin (PNAd), but not mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Most HEVs in small intestine Peyer's patches (PP) express MAdCAM-1, but not VCAM-1 and luminal PNAd. Most HEVs in mouse BALT express PNAd and VCAM-1, but not MAdCAM-1 [1,9,27]. In vivo studies from our laboratory indicate that migration of B cells and naive T cells into mouse BALT largely depends on endothelial PNAd and its lymphocyte ligand L-selectin, while migration of memory T cells into BALT requires endothelial VCAM-1 and lymphocyte α 4 integrin [9]. However, the adhesion molecules that are highly expressed on HEVs in human BALT are not known.
In this study, we evaluated BALT tissue from lobectomy specimens of 17 human adults with lung carcinoma. We found that BALT HEVs expressed PNAd and VCAM-1 but not MAdCAM-1. Most BALT B cells and some T cells expressed L-selectin and α 4 integrin. Most BALT CD4 + T cells had a memory phenotype. These results suggest that human BALT expresses endothelia and lymphocyte adhesion molecules that may be important in recruiting naive and memory/effector lymphocytes to BALT, where they may be involved in the initiation of bronchopulmonary immune responses to inhaled antigens and pathogens.

Tissue collection, frozen sectioning and histologic identification of BALT
Bronchus tissue was obtained from 49 patients who underwent lobectomy for lung carcinoma at Kagoshima Kouseiren Hospital, Kagoshima, Japan. Patients with pulmonary inflammatory disorders, such as asthma, chronic obstructive pulmonary disease, rheumatoid lung, and post-obstructive pneumonia due to tumor, were excluded from the study. Tissue samples, each ≥2 cm in maximum dimension, of bronchus and surrounding lung were taken from at least 2 nonadjacent sites in each lobectomy specimen. The sampling was carefully performed to exclude tumor. Each tissue sample was infused with a 1:1 mixture of OCT compound (Sakura Finetek, Tokyo, Japan) and phosphate-buffered saline (PBS), and frozen in OCT compound. A frozen section was cut, fixed in methanol, stained with hematoxylin and examined by light microscopy for "classic" BALT, as defined using the histologic criteria originally described and illustrated by Bienenstock and colleagues [5]. These criteria are listed in the first paragraph of the Results section. If "classic" BALT was identified on the hematoxylin-stained section, additional sections were cut, fixed in acetone, and stored at -20°C for immunostaining. If there was no BALT on the hematoxylin-stained section, we continued to cut and examine sections at 100 μm intervals until BALT was found, or the block was cut through.
Small intestine PPs, obtained from ileocecal resection specimens from 3 patients with colon carcinoma, were frozen in OCT compound. Frozen sections were cut, fixed in acetone, and stored at -20°C for immunostaining.

Antibodies and other reagents
Mouse and rat monoclonal antibodies (mAbs) used in this study are listed in Table 1. Biotin-anti-mouse IgG polyclonal antibody was purchased from eBioscience (San Diego, CA). Biotin-anti-rat IgM polyclonal antibody and peroxidase-streptavidin were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA). AEC substrate kit and Alexa Fluor 546-streptavidin were from Vector Laboratories (Burlingame, CA) and Molecular Probes (Eugene, OR), respectively.

Immunohistochemical staining
Acetone-fixed frozen sections of BALT and PP were incubated with mAb against CD31, MAdCAM-1, VCAM-1 or PNAd, or with a species-and isotype-matched negative control mAb, at room temperature for 1 hour (hr). Sections were rinsed in PBS, incubated with biotin-antimouse IgG (to detect CD31, MAdCAM-1 or VCAM-1) or biotin-anti-rat IgM (to detect PNAd) at room temperature for 30 minutes (min), rinsed in PBS, and incubated with peroxidase-streptavidin for 30 min. After a final PBS wash, slides were incubated with Vector AEC substrate (per manufacturer's instructions), stained with hematoxylin, and cover-slipped. Sections were evaluated by light microscopy.
To evaluate expression of adhesion molecules on BALT lymphocytes, sections were incubated with FITC-anti-CD3 mAb combined with PE-anti-LFA-1, PE-anti-α 4 integrin, or PE-anti-L-selectin mAb (30 min). We counted total T cells (CD3 + ) and T cells expressing LFA-1, α 4 integrin or L-selectin in BALT from 6 randomly selected patients, and calculated the percentage of T cells that expressed each adhesion molecule. In a similar manner, we determined the proportion of B cells (CD3lymphocytes in the B cell follicle) that expressed each adhesion molecule.
For each tissue, primary mAbs were replaced by conjugation-, species-and isotype-matched negative control mAbs. All slides were viewed and imaged using a confocal microscope.

Ethics
The ethics committee of the Graduate School of Medical and Dental Sciences, Kagoshima University, Japan approved this study. Each patient gave written informed consent to use tissue for research.

Statistical analysis
All data are expressed as mean ± standard derivation (SD). Chi-square test was used to evaluate the difference in the frequency of BALT between groups. P < 0.05 was considered to be statistically significant.

Detection of BALT in adult human lung
We examined hematoxylin-stained frozen sections of bronchi from lung carcinoma lobectomy specimens for BALT. We used histologic criteria that were originally proposed by Bienenstock and are extensively described in the human histopathology literature [5,28,29]. Specifically, BALT: 1) consists of lymphoid follicle(s) in the bronchial mucosa; 2) is fairly well circumscribed, without inflammation or damage of the adjacent bronchus tissue, 3) has prominent HEVs, and no or small germinal centers; 4) may contain pigment-filled macrophages, but no neutrophils or eosinophils; and 5) lacks evidence of significant epithelial injury. If BALT was identified on the hematoxylin-stained section (Fig. 1A), we immediately cut additional sections from the frozen tissue block for immunohistology staining. In some specimens, we found a few small, poorly demarcated lymphoid aggregates in the pulmonary parenchyma (Fig. 1B). These aggregates, which were not associated with a bronchus, were not analyzed in this study.
We identified BALT on hematoxylin-stained frozen sections of bronchus from 17/49 (35%) lung lobectomy specimens (Fig. 1A). BALT was identified in 40% of the lobectomy specimens from men and 26% of the specimens from women. Current smokers (42%) were more likely to have BALT than were previous smokers (25%) or non-smokers (29%). BALT was slightly more common in patients over the age of 70 years (37%) than in those 70 years or younger (32%). BALT was found in 43% of patients with squamous cell carcinoma and 31% of patients with adenocarcinomas. None of these differences were statistically significant (p > 0.05 for all factors, Chisquare test).

Adhesion molecules on BALT HEVs
To determine which adhesion molecules are expressed on BALT HEVs, we stained frozen sections of BALT with mAbs against endothelia adhesion molecules that are expressed by HEVs in secondary lymphoid tissues. These adhesion molecules include PNAd, which is highly expressed by HEVs in peripheral LNs and mouse BALT; Lymphoid tissue in human lung MAdCAM-1, which is expressed by HEVs in small intestine PPs; VCAM-1, which is highly expressed by HEV endothelia in mouse BALT; and ICAM-1 and ICAM-2, which are expressed by HEVs in LNs, PPs and mouse BALT [1,9]. For comparison to BALT, we also stained sections of PP.
There was strong expression of PNAd on all HEVs in BALT ( Fig. 2A) and on a few small vessels in PP (Fig. 2B). VCAM-1 was expressed on many HEVs in BALT, on rare HEVs in PP, and on many non-endothelial cells in BALT and PP (Figs. 2C, D). There was no staining for MAdCAM-1 on BALT HEVs in any of the patients (Fig. 2E). Bronchial venules that were not in BALT did not express PNAd, VCAM-1 or MAdCAM-1 (not shown), indicating that the bronchi were not chronically inflamed. As expected, there was strong staining for MAdCAM-1 on PP HEVs (Fig. 2F) [30]. ICAM-1 and ICAM-2 were strongly expressed on HEVs in BALT and PP (data not shown). Negative control mAbs did not stain vascular endothelia in BALT or PP sections (Figs. 2G, H).
VCAM-1 is known to be expressed by a variety of nonendothelial cells, including follicular dendritic cells and blood vessel pericytes, in human secondary lymphoid tissues [31]. Because of this extensive expression, it is difficult to determine which cells are stained with an anti-VCAM-1 mAb on single color immunohistology slides (Figs. 2C, D). To focus on VCAM-1 expression by HEV endothelia, we stained frozen sections of BALT for PNAd and VCAM-1 by two-color IF (Figs. 2I-N). Quantitative evaluation revealed that 66 ± 11% of BALT HEVs, as identified by high endothelial morphology and expression of PNAd, were PNAd + VCAM-1 + and 33 ± 11% were PNAd + VCAM-1 -(n = 5 patients).
These results indicate that the HEV adhesion molecules that are available to recruit lymphocytes into BALT differ significantly from those that are available to recruit lymphocytes into PP. Specifically, in BALT, all HEVs expressed PNAd, most HEVs expressed VCAM-1, and no HEVs expressed MAdCAM-1. In PP, all HEVs expressed MAd-CAM-1 and a few small HEVs expressed PNAd or VCAM-1.

Lymphocytes in BALT
We used two-color frozen section IF staining to determine which lymphocyte subsets are present in BALT, and whether certain subsets occupy specific microenvironments within the tissue. Our stains revealed well demarcated B (CD19 + ) and T (CD3 + ) cell zones (Fig. 3A). The B cells formed central aggregates that were surrounded by T cells, most of which expressed CD4 (Figs. 3A and 3B). CD45RO (memory marker) and CD45RA (naive marker) were expressed on 86 ± 2% and 9 ± 2% of the CD4 + T cells (n = 7 patients), respectively (Figs. 3C and 3D).

Discussion
Migration of lymphocytes from blood vessels into tissues is a complex process with multiple adhesion and activation steps [1,32]. Briefly, lymphocytes transiently adhere to the endothelial surface of venules in the tissue (step 1), are activated by chemokines (step 2), and adhere firmly to the endothelia (step 3), before migrating through the vessel wall into the tissue [1,32]. Thus, the adhesion molecules that are expressed on the HEVs in a secondary lymphoid tissue are important in determining which subsets of lymphocytes migrate into the tissue. Here we evaluated BALT from lobectomy specimens of 17 human adults with lung carcinoma for the expression of HEV and lymphocyte adhesion molecules that might recruit lymphocytes from blood vessels into BALT. We found that BALT HEVs expressed PNAd, ICAM-1 and ICAM-2, with or without VCAM-1. MAdCAM-1 was not expressed on HEVs in any of the BALT samples. The adhesion molecule profile of HEVs in human BALT is similar to that of HEVs in mouse BALT, differing only in the extent of VCAM-1 expression (66% of HEVs in human and 91% of HEVs in mouse BALT) [9]. Thus, BALT in humans, as in mice [9], has HEV adhesion molecules that are capable of recruiting distinct subsets of lymphocytes from the bloodstream into the tissue.
As in mice, the combination of adhesion molecules on HEVs in human BALT differs from that on HEVs in other secondary lymphoid tissues [9]. Specifically, VCAM-1, which was expressed by endothelia of 66% of BALT HEVs in our study, is not significantly expressed on HEV endothelia in PP (Fig. 2D), adenoids and peripheral LNs [31,33]. PNAd, which was strongly expressed on all HEVs in BALT, is weakly expressed on HEVs in appendix and PP (Fig. 2B) [34]. MAdCAM-1, which was not expressed on BALT HEVs, is strongly expressed on appendix and PP HEVs [30].
We found that 86% of BALT CD4 + T cells had a memory phenotype (CD4 + CD45RO + ). Since BALT does not have afferent lymphatic vessels [6], these memory T cells may  The Stamper-Woodruff in vitro binding assay can be used to determine which adhesion molecules mediate the binding of viable lymphocytes to HEVs on frozen sections of tissues [12,35]. Due to the scarcity of frozen samples of human BALT, we were unable to perform these assays. However, our immunohistology studies on human and mouse BALT and our functional studies on mouse BALT [9] suggest that PNAd and VCAM-1 are involved in organselective recruitment of specific subsets of lymphocytes to human BALT. Specifically, L-selectin + naive T cells could be recruited from the bloodstream into human BALT by binding to PNAd + HEVs [36]. Since specialized cells, such as M cells and/or dendritic cells, transport airway luminal antigens into BALT, the naive T cells could meet antigenbearing dendritic cells in BALT, resulting in the generation of lung-specific α 4 β 1 integrin + memory T cells. Following release into the bloodstream, the α 4 β 1 integrin + memory T cells could be recruited back to BALT by binding to VCAM-1 + HEVs. Additionally, the α 4 β 1 integrin + memory T cells could migrate into inflamed lungs by binding to VCAM-1, which is highly expressed on vessels in human inflamed lung [37][38][39]. Thus, in humans as in mice, the VCAM-1/α 4 β 1 integrin adhesion system may unify the migration pathways of T cells into BALT and inflamed lung.
The frequency of BALT in normal lungs of healthy adults varies considerably between species. For example, BALT is found in lungs of most normal adult rats, rabbits and guinea pigs [5,6,10,12,13,40]. In contrast, the existence of BALT in lungs of healthy adult humans is controversial. In published reports, BALT was found in 0% to 74% of normal human lungs, as compared to 35% of lungs in our study [14][15][16]18,20,41]. The marked differences in the frequency of human BALT between studies may be due to several factors, including the criteria used to determine if the lung is "normal" and/or the person is "healthy", differences in human subject populations, the source and size of the specimen (i.e., bronchial biopsy, surgical wedge resection, or surgical resection of a lobe or lung), the number of tissue samples and sections examined, and the histologic criteria used to identify BALT.
As with other lymphoid tissues, BALT can undergo marked changes in size, cellular composition, and function during local immune responses. In animal models, BALT can be induced and/or activated by infection or immune stimulation of the lower respiratory tract [23,[42][43][44][45]. In humans, hyperplastic BALT can be seen in patents with chronic pulmonary inflammatory disorders, such as hypersensitivity pneumonitis, and in patients with autoimmune diseases, including rheumatoid arthritis and Sjogren's syndrome [15,[46][47][48][49][50]. Exposure to irritants, such as those in cigarette smoke, may influence the development of BALT, as BALT was found in 82% of smoking and 14% of non-smoking human adults in one study [18]. In our study, BALT was slightly more common in current smokers (42%) than in non-smokers (29%).
It is clear from this study and previous studies that human BALT shares many features with secondary lymphoid tissues, including distinct T cell and B cell zones, follicular dendritic cells, PNAd + HEVs, and expression of lymphoid chemokines CXCL13 and CCL21 (Fig. 2) [49]. Some unique features of BALT in humans and animals, however, lead to the debate regarding whether BALT is a secondary lymphoid tissue controlled by a precise developmental program or a tertiary lymphoid tissue controlled by lymphoid neogenesis. BALT varies by species, strain, age, and antigen stimulation: it is more common in rabbits and rats than in mice [5,6,10,13,40]; autoimmune-prone nonobese diabetic mice and old mice have more prominent BALT than nonautoimmune-prone and young mice, respectively [9]; and antigen stimulation and microbe infection can induce BALT formation in lungs of mice [23,42,43,51]. Additionally, HEVs in mouse and human BALT express VCAM-1 (Fig. 2) [9], which is frequently seen on HEVs of tertiary lymphoid tissues but not of LNs and PPs [52]. Thus, BALT is not identical to "conventional" secondary lymphoid tissues such as LNs and PPs.
A limitation of our study and of several other studies of human bronchopulmonary immunology [35,53] is that the tissues were obtained from carcinoma lobectomy specimens. The factors, such as cigarette smoke, that led to the development of the carcinoma could also lead to the development of chronic inflammation, such as chronic bronchitis. In addition, the carcinoma could initiate an inflammatory response (for example, by obstructing a bronchus, leading to post-obstructive pneumonia). We made every effort to minimize these potential confounding factors. Specifically, we excluded patients with 1) known inflammatory pulmonary diseases; 2) macroscopic evidence of inflammation or infection in the lobectomy specimen; or 3) histologic features of inflammation or infection [29]. Moreover, we used strict histopathologic criteria to identify "classic" BALT as described by Bienenstock and colleagues and in the pathology literature [5,29].

Conclusion
In summary, we found that the HEVs in human BALT express a unique set of adhesion molecules that are very similar to those expressed in mouse BALT but markedly different from those expressed in other human secondary lymphoid tissues. The adhesion molecules on human BALT HEVs may be important in recruiting specific subsets of naive and memory/effector lymphocytes to BALT, where they may be involved in the development of bronchopulmonary immune responses to inhaled antigens and pathogens. Thus, BALT may be involved in the induction or/and modification of physiologic and pathologic bronchopulmonary immune responses. Selective targeting of PNAd/L-selectin-and VCAM-1/α 4 β 1 integrin-mediated lymphocyte migration pathways might serve as a novel strategy for treating inflammatory bronchopulmonary diseases. Publish with Bio Med Central and every scientist can read your work free of charge