Fig. 5
From: Submersion and hypoxia inhibit alveolar epithelial Na+ transport through ERK/NF-κB signaling pathway
Hypoxia-induced decrease of α-ENaC was mediated by the activation of the ERK1/2 signaling pathway. A, B Representative and statistical data of Western Blot of α-ENaC expression of A549 cells cultured under hypoxia (8 ml) for 24 h after PD98059 (10 µM) treatment. D, E Representative and statistical data of Western Blot of α-ENaC expression of AT2 cells cultured in hypoxic condition (8 ml) for 24 h after PD98059 (10 µM) treatment. C, F qRT-PCR results for α-ENaC mRNA in A549 and AT2 cells, and β-actin was used as internal reference. *P < 0.05, **P < 0.01, compared with Control group; &P < 0.05, compared with Hypoxia group. One-way ANOVA followed by Bonferroni’s test was used to analyze the difference of the means for significance. n = 3–6