Fig. 2

Hypoxia decreased the protein and mRNA of ENaC. A Representative graph of α/γ-ENaC in different volumes (2 ml, 4 ml, 6 ml, and 8 ml) of culture medium for 24 h in A549 cells. B, C The statistical data of α/γ-ENaC in different volumes (2 ml, 4 ml, 6 ml, and 8 ml) of culture medium for 24 h in A549 cells obtained from Western Blot and quantified through gray analysis (α/γ-ENaC/β-actin). D Representative graph of α/γ-ENaC in 8 ml culture medium at different times (12 h, 24 h, and 48 h) in A549 cells. E, F The statistical data obtained from Western Blot and quantified through gray analysis (α/γ-ENaC/β-actin). G–I α/β/γ-ENaC mRNA expression level of A549 cells after 12 h, 24 h, and 48 h in 8 ml culture medium (α/β/γ-ENaC/β-actin). ^&P < 0.05, compared with 2 ml group. *P < 0.05, **P < 0.01, ***P < 0.001, compared with 0 h group. One-way ANOVA followed by Bonferroni’s test was used to analyze the difference of the means for significance. n = 3–6