Fig. 4

Effects of bronchoalveolar lavage fluid from AC patients on NSCLC cell migration. H1299 human NSCLC cell line with high metastasis potential was seeded into a 24-well transwell plate. At 24 h after seeding, bronchoalveolar lavage (BAL) fluid (50 µL) from AC patient 1# was added to the cell culture well. Vancomycin (2.5 µg/L) and kanamycin (5 µg/L) were also added for BAL + vancomycin group and BAL + kanamycin group, respectively. At 48 h after BAL and antibiotic management, migrated cells were stained (A) and counted (B). (C) Schematic representation of abundance variation of lung mucosa-colonizing bacteria communities and surrounding immune cells in different NSCLC stage. Left pane: lung mucosa-colonizing bacteria of healthy population were basically divided into four communities (Community 1, 2, 3, 4); Middle pane: member abundances of most communities, except community 2, increase in NSCLC patients; Right pane: most members of community 2 further increase their abundances in NSCLC patients with metastasis, and they positively correlate with immune cells, such as eosinophils, neutrophils and monocytes