Fig. 2
From: Use of a pulmosphere model to evaluate drug antifibrotic responses in interstitial lung diseases
Fibronectin expression in increased in ILDs and correlates with pulmosphere invasiveness. A IHC staining showing expression of fibronectin in formalin-fixed, paraffin-embedded tissue section of a representative of 9 control subjects and 5 subjects with ILD. Scale bars: 100 μm. B Scatter plot of DAB fibronectin-EDA intensity control vs ILD tissue depicting significantly higher intensity in the ILD tissues. N = 9 for control subjects and 17 for ILD (Control Vs ILD, 0.075 ± 0.020 vs 0.122 ± 0.033, **P value = 0.0012 estimated by Mann–Whitney test). C Simple linear regression analysis of ZOI% (invasiveness) with fibronectin expression showing positive correlation, P value < 0.001, R2 = 0.88, r = 0.94. D RT-PCR showing col1A1 mRNA expression normalized to β-actin among Control Vs ILD pulmospheres,0.73 ± 0.06 vs 0.97 ± 0.31, P value = 0.0476 estimated by Mann–Whitney test. E Simple linear regression analysis of ZOI% (invasiveness) with collagen expression showing positive correlation, P value = 0.031, R2 = 0.45, r = 0.68