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Fig. 6 | Respiratory Research

Fig. 6

From: Inhibition of miR-4640-5p alleviates pulmonary hypertension in chronic obstructive pulmonary disease patients by regulating nitric oxide synthase 1

Fig. 6

Inhibition of miR-4640-5p ameliorates hypoxia-induced PAH in rat. Sprague–Dawley rats were randomly divided into four groups (n = 5): Normoxia group, Hypoxia group, Hypoxia + anti-NC group, Hypoxia + anti-miR-4640-5p group. Pulmonary hypertension model was established by exposing the rats in Hypoxia group, Hypoxia + anti-NC group and Hypoxia + anti-miR-4640-5p group to 10% O2 for 8 h per day for 4 weeks. After terminal harvest, the relative expression of miR-4640-5p (A), NOS1 mRNA (B), and NOS1 protein (C) in rat lung tissues was analyzed. DG After the rats were anesthetized with 30 mg/kg sodium barbital, the abdominal incision was made through the xiphoid process, and the scalp needle was inserted into the right ventricle from the abdominal cavity through the diaphragm to measure and calculate the (D) RVSP, (E) RV/(LV + S), (F) CO, and (G) RVSP/CO. H The protein expression of Drp1, FIS1, MFN1, NOS1 and GAPDH in PASMCs was analyzed by western blot. Western blot experiments were independently repeated for at least three times *p < 0.05, **p < 0.01, ***p < 0.001

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