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Fig. 4 | Respiratory Research

Fig. 4

From: Circ-Ntrk2 acts as a miR-296-5p sponge to activate the TGF-β1/p38 MAPK pathway and promote pulmonary hypertension and vascular remodelling

Fig. 4

MiR-296-5p overexpression alleviates pulmonary vascular remodelling and improves PAH. a Agomir constructed for miR-296-5p was used to upregulate miR-296-5p. All treatment groups received injections twice weekly for modelling for 21 days. b Echocardiographic measurements and images illustrating pulmonary artery acceleration time (PAAT) and pulmonary arterial velocity time integral (PAVTI) in the NOR, HYP, HYP + Agomir-NC, and HYP + Agomir groups are shown (n = 5). c Right ventricular systolic pressure (RVSP) and the right ventricle (RV)/left ventricle (LV) + septum (S) weight ratio in the NOR, HYP, HYP + Agomir-NC, and HYP + Agomir groups (n = 5). d Analysis of pulmonary artery remodelling was performed using H&E staining and immunofluorescence (n = 5). e Detection of collagen deposition around blood vessels using Masson staining (up) (n = 5) and H&E staining of myocardial cells in right ventricular wall (bottom) (n = 5). f Analysis of PCNA (proliferating cell nuclear antigen) and cyclin D1 expression in the lung tissues (n = 5) and PASMCs (n = 6). The gel or blot images have been cropped and the complete images have been submitted separately (Additional file 4). g EdU in PASMCs (n = 6). h CCK-8 assay in PASMCs (n = 6). All values are presented as the mean ± SD. *P < 0.05, **P < 0.01

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