Fig. 3

Circ-Ntrk2 acts as a sponge for miR-296-5p. a miRanda bioinformatics software predicted miRNAs that could bind to circ-Ntrk2. b Quantitative polymerase chain reaction (qPCR) analysis of miR-296-5p expression in mouse lung tissues and PASMCs (n = 6). c PASMCs were transfected with si-circ-Ntrk2, and qPCR was used to detect miR-296-5p expression (n = 6). d Fluorescence in situ hybridization (FISH) was used to observe the colocalization of circ-Ntrk2 and miR-296-5p. e TargetScan predicted binding sites and secondary structures of circ-Ntrk2 and miR-296-5p. f Dual luciferase vector and mutant plasmids were designed based on binding sites between circ-Ntrk2 and miR-296-5p. Dual luciferase assays were used to validate the interactions between circ-Ntrk2 and miR-296-5p (n = 6). All values are presented as the mean ± SD. *P < 0.05, **P < 0.01