Fig. 8From: Inhibition of fatty acid synthase protects obese mice from acute lung injury via ameliorating lung endothelial dysfunctionInhibition of FASN decreased endothelial inflammation in MLECs under metabolic stress when exposed to LPS. a–d The mRNA level of TNF-α, IL-6, and IL-1β in each group of MLECs were measured by qPCR (a, c). The secretion levels of TNF-α, IL-6, and IL-1β in the cell supernatant were measured by ELISA assay in each group of MLECs (b, d). One-way ANOVA and LSD or Games–Howell posthoc analysis. e, f The secretion levels of TNF-α, IL-6, and IL-1β in the cell supernatant were measured by ELISA assay in MLECs with FASN shRNA or NC shRNA under PA (e) or adipocytes (f) treatment when exposed to LPS. Student’s t test analysis. n = 3 biologically independent experiments. Data were represented as mean ± SDBack to article page