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Fig. 3 | Respiratory Research

Fig. 3

From: Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets

Fig. 3

Protein disulfide isomerases clustered to fraction 5. A Levels of AGR2 in fraction 1–5 from unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. There was more AGR2 in fraction 5 than 4 in stimulated WT tracheas, *P = 0.04, Mann–Whitney test. B Immunostaining of paraffin sections from newborn WT piglets demonstrating that AGR2 (red) was produced in submucosal gland epithelial cells. Dashed line outlines the submucosal gland. Scale bar: 10 µm. C Levels of PDIA1 in fraction 1–5 from unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. There was more PDIA1 in fraction 5 than 4 in stimulated WT tracheas, **P = 0.004, Mann–Whitney test. D Levels of PDIA3 in fraction 1–5 from unstimulated (open circles, n = 4) and stimulated (filled circles, n = 6) WT piglets. There was more PDIA3 in fraction 5 from stimulated WT piglets than in fraction 1 from the same piglets, n = 6, **P = 0.002. E Immunostaining of paraffin sections from newborn WT piglets demonstrating that PDIA3 (red) was produced in submucosal gland MUC5B producing epithelial cells (green). Scale bar: 10 µm. F The antibacterial protein lactoferrin clustered to fraction 5. Normalized intensities in unstimulated WT piglets (green) denoted by open circles (n = 4 piglets) and stimulated by filled circles (n = 6 piglets) and CF piglets (pink) denoted by open circles (n = 3 piglets) and stimulated by filled circles (n = 3 piglets). G There was more lactoferrin in fraction 5 compared to fraction 1 when all values were pooled (WT and CF, unstimulated and stimulated, n = 16 in each group), *P = 0.03, Mann–Whitney test. Each data point in A, C, D, F, G represents one piglet and results are presented with median with interquartile range. Scale bar in C and E 10 µm

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