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Fig. 8 | Respiratory Research

Fig. 8

From: Azithromycin ameliorated cigarette smoke-induced airway epithelial barrier dysfunction by activating Nrf2/GCL/GSH signaling pathway

Fig. 8

Role of macrolides on airway epithelial barrier dysfunction in CSE-incubated HBECs. HBECs were pre-incubated with or without different concentrations of EI (50, 5, 0.5 μM) or SPI (50, 5, 0.5 μM) for 1 h, and subsequently co-incubated with 3% CSE for 24 h. A, B The levels of IL-6 and GSH and were measured by relevant commercial assay kits. C The TEER was monitored for 24 h at specified time points in HBECs. D Flow cytometry assay was used to detect the apoptosis of HBECs. E, F The expressions of ZO-1 in HBECs were detected by immunofluorescence staining. The scale bar represents 50 μm. G The expression of Nrf2 was detected by western blot analysis. Data shown are means ± SD, n = 6. ##P < 0.01 and ###P < 0.001 vs control group;*P < 0.05, **P < 0.01, ***P < 0.001 and ns means no significant difference vs CSE group

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