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Fig. 7 | Respiratory Research

Fig. 7

From: Azithromycin ameliorated cigarette smoke-induced airway epithelial barrier dysfunction by activating Nrf2/GCL/GSH signaling pathway

Fig. 7

Knockout of Nrf2 deprived the effects of AZI on airway epithelial barrier dysfunction in vivo. A, B The levels of IL-6 and TNF-α in the BALF of mice were analyzed by ELISA. C, D The content of GSH and the activity of GCL in lung tissues were measured by relevant assay kits. E, F The contents of l-Glutamic acid and pyroglutamylglycine in lung tissues were measured by LC–MS. G The expressions of E-cadherin and ZO-1in lung tissues were observed by immunohistochemical staining. The scale bar represents 100 μm. H, I The expressions of proteins relevant to airway epithelial barrier dysfunction were detected by western blot analysis. Data shown are means ± SD, n = 6. #P < 0.05, ##P < 0.01, *P < 0.05, **P < 0.01, ***P < 0.001 and ns means no significant difference, comparison between groups as indicated in the figure

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