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Fig. 6 | Respiratory Research

Fig. 6

From: Azithromycin ameliorated cigarette smoke-induced airway epithelial barrier dysfunction by activating Nrf2/GCL/GSH signaling pathway

Fig. 6

Knockdown of Nrf2 abolished the effects of AZI on airway epithelial barrier dysfunction in vitro. After pre-incubated with or without AZI (50 μM) for 1 h, HBECs with Nrf2 knockdown were co-incubated with 3%CSE for another 24 h. A The interference efficiency was determined by Western blot. B The levels of IL-6 and TNF-α in the supernatant of HBECs were analyzed by ELISA. C, D The contents of GSH and ROS along with the activities of GCL and GS were measured by commercial assay kits. E The contents of L-Glutamic acid and pyroglutamylglycine were measured by LC–MS. F The TEER was monitored for 24 h at specified time points in HBECs. G, H Western blot analysis was performed to examine the expressions of indicated proteins in HBECs. Data shown are means ± SD, n = 6. #P < 0.05, ##P < 0.01, ###P < 0.001, *P < 0.05, **P < 0.01 ***P < 0.001 and ns means no significant difference, comparison between groups as indicated in the figure

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