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Fig. 6 | Respiratory Research

Fig. 6

From: IL-27 induces autophagy through regulation of the DNMT1/lncRNA MEG3/ERK/p38 axis to reduce pulmonary fibrosis

Fig. 6

DNMT1 overexpression or MEG3 silencing reversed IL-27-activated autophagy to attenuate TGF-β1-induced fibrosis in MRC-5 cells. A, B RT‒qPCR for DNMT1 and MEG3 transfection efficiency; C EdU for cell proliferation viability (magnification: × 200; scale bars: 100 μm); D Western blot for protein expression levels; E Immunofluorescence for LC3 and Beclin1 fluorescence intensity (magnification: × 200; scale bars: 10 μm); F–H The mRNA levels of α-SMA, FN, and COL I were detected by RT‒qPCR; I Western blot to detect α-SMA, FN, and COL I protein expression levels; J Immunofluorescence to detect α-SMA, FN, and COL I fluorescence intensity (magnification: × 200; scale bars: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001; values are expressed as the mean ± standard deviation (n = 3)

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