Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: IL-27 induces autophagy through regulation of the DNMT1/lncRNA MEG3/ERK/p38 axis to reduce pulmonary fibrosis

Fig. 5

IL-27 upregulates MEG3 expression by inhibiting DNMT1-mediated methylation of the MEG3 promoter. A Detecting methylation in MRC-5 cells by MSP; B Detecting MEG3 mRNA levels in different treatment groups by RT‒qPCR; C, D Detecting DNMT1 expression in different treatment groups by RT‒qPCR and Western blotting; E RT‒qPCR to detect MEG3 expression levels; F chromatin immunoprecipitation to detect DNMT1 enrichment in the MEG3 promoter region. *P < 0.05, **P < 0.01, ***P < 0.001; values are expressed as the mean ± standard deviation (n = 3)

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us