Fig. 3

IL-27 attenuates TGF-β1-induced fibrosis in MRC-5 cells by activating autophagy through inhibition of the ERK/p38 signaling pathway. A Western blot for ERK and p38 phosphorylated protein levels; B EdU for cell proliferation viability (magnification: × 200; scale bars: 100 μm); C Detecting the level of autophagy-related markers LC3 and Beclin1 by Western blot; D Immunofluorescence for LC3 and Beclin1 fluorescence intensity (magnification: × 200; scale bars: 10 μm); E–G Detecting the mRNA levels of α-SMA, FN and COL I by RT‒qPCR; H: Western blot for α-SMA, FN and COL I protein levels; I: Immunofluorescence for α-SMA, FN and COL I fluorescence intensity (magnification: × 200; scale bars: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001; values are expressed as the mean ± standard deviation (n = 3)