Fig. 2

IL-27 attenuates TGF-β1-induced fibrosis in MRC-5 cells through activation of autophagy. A EdU for cell proliferation viability (magnification: × 200; scale bars: 100 μm); B Western blot detection of autophagy-related markers LC3 and Beclin1 protein expression levels; C Immunofluorescence detection of LC3 and Beclin1 fluorescence intensity (magnification: × 200; scale bars: 10 μm); D–F RT‒qPCR detection of α-SMA, FN, and COL I mRNA expression levels; G Western blot for α-SMA, FN, and COL I protein expression levels; H Immunofluorescence for α-SMA, FN, and COL I fluorescence intensity (magnification: × 200; scale bars: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001; values are expressed as the mean ± standard deviation (n = 3)