Fig. 6

DC260126 alleviates AHR in obese asthmatic mice through RhoA/ROCK1 signal pathway. A Within 24 h after last challenge, airway resistance was measured by inhalation of methacholine (0 to 50 mg/ml) to determine airway resistance (n = 6 per group). B Severity of inflammation cell infiltration in the airways was assessed by H&E staining, and semi-quantitative pathology scores were shown. Mouse lung tissues from different treatment groups were harvested, the protein expression of GPR40 (C), the activity of RhoA (GTP-RhoA) (D) and protein expression ROCK1 (E) were evaluated by western blot (n = 4 per group). The data were presented as the mean ± S.E.M. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control, ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 compared with the HDF-OVA group. The cell lysates of HASM cells from different treatment groups were harvested, the activity of RhoA (GTP-RhoA) (F) and protein expression of ROCK1 (G) were assessed by western blot (n = 4 per group). The data were presented as the mean ± S.E.M. *P < 0.05, **P < 0.01 compared with the control, ^##P < 0.01 compared with the OA-treated group