Fig. 1
GPR40 expression is elevated in the lung tissues of obese asthmatic mice. A The mice were fed with normal chow diet or HFD for 12 weeks, followed by sensitized with OVA that emulsified in Al (OH)3 gel on day 1 and 13 from the 12th week, and then challenged with aerosolized OVA for 0.5 h a day from day 25 to 31. DC260126 was given to HFD-OVA model mice daily by i.p. injection at 0.5 h before each challenge, vehicle (0.1% DMSO) was given to the mice in control, OVA, HFD, and HFD-OVA instead. B The GPR40 expression in the pulmonary tissues of mice was assessed by immunohistochemical staining. The data were represented as the mean ± S.E.M. *P < 0.05, and **P < 0.01 compared with the control group. C FFAs level in serum was assessed by ELISA. D The body weight in lean mice (Control, OVA group) and obese mice (HFD, HFD-OVA group) was recorded every three weeks, the results were expressed as the percentage of body weight gain. E Mice body weight was measured with (HFD + OVA + DC260126 3 mg/kg, HFD + OVA + DC260126 10 mg/kg group) or without (Control, OVA, HFD, HFD-OVA group) DC260126 administration during 7 consecutive days. The data were represented as the mean ± S.E.M. (n = 6 per group). **P < 0.01 and ***P < 0.001 compared with the control group. #P < 0.05 compared with the HFD-OVA group