Fig. 6

BRD4 inhibitor JQ1 suppresses IL-6 and KC expression in the lung of mice during viral infection after CS exposure. Mice were injected with BRD4 inhibitor JQ1 or vehicle control once a week from the first day of cigarette smoke (CS) exposure. After CS exposure for 12 weeks, mice were then infected with influenza virus or vehicle control. They were sacrificed and evaluated on day 7 after infection. The total leukocyte counts in bronchoalveolar lavage fluid (BALF) (A), the relative mRNA levels (fold change) of IL-6 (B) and KC (C) in the lung, the protein levels of IL-6 (D) and KC (E) in BALF are shown (n = 5 mice/group). CS + Flu: CS-exposed mice with influenza virus infection; *P < 0.05, **P < 0.01