Table 1 Sequences of oligonucleotides for forward and reverse primers for real-time RT-PCR
From: Vascular and pulmonary effects of ibuprofen on neonatal lung development
Gene product | Forward primer | Reverse primer |
|---|---|---|
IL6 | 5′-ATATGTTCTCAGGGAGATCTTGGAA-3′ | 5′-TGCATCATCGCTGTTCATACAA-3′ |
CINC1 | 5′-GCACCCAAACCGAAGTCATA-3′ | 5′-GGGGACACCCTTTAGCATCT-3′ |
MCP1 | 5′-ATGCAGTTAATGCCCCAGTCA-3′ | 5′-TTCTCCAGCCGACTCATTGG-3′ |
TF | 5′-CCCAGAAAGCATCACCAAGTG-3′ | 5′-TGCTCCACAATGATGAGTGTT-3′ |
β-Actin | 5′-TTCAACACCCCAGCCATGT-3′ | 5′-AGTGGTACGACCAGAGGCATACA-3′ |