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Fig. 2 | Respiratory Research

Fig. 2

From: Vascular and pulmonary effects of ibuprofen on neonatal lung development

Fig. 2

Bubble plot of pathways significantly affected by 500 µM of ibuprofen (A, N = 4–5). Representative photos of cell cycle analysis of HUVECs treated with DMSO (0.1%, v/v), 100, 500 or 1000 µM of ibuprofen (B) and proportion of cells at different cell cycle stages, G0/G1, S and G2/M (C, N = 3). Representative photos and fluorescence intensity quantification of the DNA damage marker, 8-hydroxy-2′-deoxyguanosine (8-OHdG) staining in HUVECs treated with DMSO or ibuprofen (D and E, N = 5). Representative photos and quantification of apoptosis analysis by propidium iodide (PI) and Annexin V staining in HUVECs treated with DMSO or ibuprofen (G and F, N = 3). Representative photos of TUNEL staining of neonatal rat lung tissue exposed to ibuprofen. Rat pups were kept in room air (RA) and were daily injected subcutaneously with arginine buffer (RA) or ibuprofen (50 mg/kg/day; RA-IB) until 10 days of age (H). DMSO, open circles; 100 µM ibuprofen, green circles; 500 µM ibuprofen, blue triangles; and 1000 µM ibuprofen, red squares. Values are expressed as mean ± SD (C and F) or mean ± SEM (E). *p < 0.05, **p < 0.01 and ***p < 0.001 versus DMSO controls

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