Fig. 5
From: The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis
The effects of the ST2-Fc fusion protein on IL-33 and ST2 in vivo. ELISA analysis of IL-33 in the BALF (A). qPCR analysis of lung IL33 mRNA levels with all values normalised to saline isotype control (B). ELISA analysis of ST2 levels in the BALF (C) and lung tissue (D). qPCR analysis of IL1RL1 (ST2) gene expression in the lungs with all values normalised to saline-treated isotype control mice (E). Assessment of cross-reactivity between the ST2-Fc fusion protein and the ST2 ELISA kit (F). For Figures B & D, bars indicate median values and statistical analysis was performed by Mann–Whitney U test. For Figures D & E bars indicate mean values and statistical analysis was performed by Two-way ANOVA with Tukey’s multiple comparisons test. For A–E, each data point represents a single mouse. n = 6–8 per treatment group. For F, data is presented as mean ± SD