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Fig. 1 | Respiratory Research

Fig. 1

From: The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis

Fig. 1

IL-33 expression in ILD lung tissue and non-IPF and IPF lung fibroblasts. IL-33 expressing cells in ILD (n = 43) and non-ILD (n = 4) lung tissue were identified via IHC. Representative images of IL-33 staining in non-ILD controls at × 10 magnification (A) and ILD samples at × 10 magnification (B) and × 20 magnification (C, D) are shown. Basal IL33 gene expression in IPF (n = 5) and non-IPF (n = 5) human lung fibroblasts (HLFs) was measured by qPCR and normalised to the average ΔCt value calculated for non-IPF cells. Bars indicate median values and each data point represents a single donor. Statistical analysis was performed by Mann–Whitney U test (E). 20 μg protein/lane was separated by SDS-PAGE and basal IL-33 protein expression by non-IPF (n = 3) and IPF (n = 4) HLFs assessed by western blot (F) and quantified relative to α-Tubulin via densitometry (G). A representative cropped western blot is shown with the whole IL-33 blot viewable in Additional file 1: Fig. S1. Bars indicate mean values and each data point represents a single donor with statistical analysis performed by Unpaired t-test

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