Skip to main content
Fig. 1 | Respiratory Research

Fig. 1

From: RAGE inhibition alleviates lipopolysaccharides-induced lung injury via directly suppressing autophagic apoptosis of type II alveolar epithelial cells

Fig. 1

Activated RAGE signals and increased inflammation is characteristic of LPS-induced lung injury. A Chest radiograph of mice treated with LPS through nasal inhalation. B Slices of mice lungs were prepared. Representative HE staining images of mouse lung sections showing injury after LPS stimulation at different time points, images × 40 magnification, scale bar 50 μm. The apoptosis of lung cells was evaluated by the TUNEL assay and images were obtained by spinning disk confocal microscopy at × 40 magnification (scale bar 50 μm) and × 200 magnification (scale bar 10 μm). C Serum IL-6 and IL-8 concentrations were determined by indicated ELISA kit. D The total protein levels of BALF in LPS-induced lung injury mice. E The levels of IL-6, IL-8 and sRAGE in BALF of mice upon LPS treated at different time points. F Immunochemistry of RAGE expression in the mice lung sections under the stimulation of LPS. Images × 40 magnification, scale bar 50 μm. G The immunoblot of RAGE in lung tissue was performed and its expression increased over time, n ≥ 3 examinations. H The relationship of IL-6 and IL-8 with sRAGE in the BALF was performed by Pearson’s correlation analysis. *Indicates the significant difference compared with the control group (0 h), p < 0.05, differences in characteristics between groups were analyzed using the Kruskal–Wallis test with Dunn’s post hoc tests

Back to article page