Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: Activation of NLRP3 inflammasome in lung epithelial cells triggers radiation-induced lung injury

Fig. 5

NLRP3 inflammasome is activated and promoted the progression of RPF in vivo. A Western blotting showing that NLRP3 protein level and GSDMD cleavage is increased in lung tissue of mice 7 days after 16 Gy thoracic X-ray radiation (n = 4). B ELISA results of IL-1β in BALF and serum of mice at different time points after radiation (n = 5). C Representative images of IHC staining for NLRP3 in lung tissue of mice at different time points after radiation (n = 5). D Representative images of NLRP3 and Masson’s staining in lung tissue of mice 2 and 5 months after radiation (n = 5). E Flow cytometry of the expression levels of CD11b in lung tissue of mice to confirm the depletion efficiency of macrophage (n = 5). F Representative images of IHC staining for NLRP3 in lung tissue of mice receiving macrophage depletion at different time point after radiation (n = 5). G ELISA results of IL-1β in BALF and serum of mice in different groups at different time points after radiation (n = 5). H Representative images of lung tissue and Masson’s staining of lung tissue of wild-type and IL-1R−/− mice (n = 5). I Representative images of Masson’s staining in lung tissue of mice treated with or without MCC950 5 months after radiation (n = 5). Bar graphs show the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. RPF radiation-induced pulmonary fibrosis, ELISA enzyme-linked immunosorbent assay, IL interleukin, BALF bronchoalveolar lavage fluid, IHC immunohistochemical, WT wild type, 1L-1R IL-1 receptor, SEM standard error of mean, ns not significant

Back to article page