Fig. 6

Molecular effect of intraluminal chloride and GsMTx4 in smooth muscle cells. a-c Upper panel represents the main cumulative effect of intraluminal chloride concentration ([Cl⁻]) and medium supplementation with and without GsMTx4. a Examples of representative blots are shown. b, c Protein expression levels for b myosin light chain 2 (MLC2), and c alpha-smooth muscle actin (α-SMA) are quantified. d–f Lower panel shows the additional effect of PIEZO1/2 inhibition after intraluminal injection of Cl⁻ channels inhibitors: cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR and; calcium-dependent Cl⁻ channel inhibitor A01 (CaCCinh) to CaCCs. d Examples of representative blots are shown. e–f Relative expression levels of e MLC2, and f α-SMA are displayed. 143 mM Cl⁻ and standard solution (SS) represent the control condition for [Cl⁻] and Cl⁻ channels inhibitors, respectively. White and dotted rectangles represent the medium supplementation with and without GsMTx4, respectively. Each lane represents a pooled-tissue sample, and the relative expression levels were determined against GAPDH. n ≥ 4 were used per antibody/condition. Results are presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the two-way ANOVA. p < ^α0.0001, ^β0.001, ^γ0.01, ^µ0.05