Fig. 3

Intraluminal chloride modulates the expression of ghrelin, bombesin, PIEZO1, and PIEZO2. a–e Upper panel represents the main effects of the distinct chloride concentrations ([Cl⁻]). a Examples of representative blots are shown. b–e Relative expression levels of b ghrelin, c bombesin, d PIEZO1, and e PIEZO2. f–j Lower panel shows the molecular effect of intraluminal Cl⁻ channels inhibition by anthracene-9-carboxylic acid (A9C) to transmembrane protein 16A (TMEM16A); cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR and calcium-dependent Cl⁻ channel inhibitor A01 (CaCCinh) to CaCCs. f Examples of representative blots are shown. g–j Protein expression levels for g ghrelin, h bombesin, i PIEZO1 and j PIEZO2. Black rectangles define the control group. Each lane represents a pooled-tissue sample, and relative expression levels were determined against GAPDH. n ≥ 4 were used per antibody/condition. Results are presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the one-way ANOVA. p < ^α0.0001