Fig. 5
From: Detection of early-stage lung cancer in sputum using automated flow cytometry and machine learning
Model variables and sample quality metrics from FCM. A Region R3 (shaded) counts represent viable singlets with high TCPP signal relative to side scatter (log10-transformed). Compare A to unstained control tube in B. C Events in region R2 (shaded) are below the viability threshold but nevertheless have relatively high FVS510 signal relative to forward scatter (log10-transformed). Compare C to unstained control tube in D. E The tan-shaded area contains non-macrophage (CD206low) leucocytes (CD66b/CD3/CD19mid). The area shaded in green (CD206mid/high CD66b/CD3/CD19low−mid) contains lung macrophages required for the sample to be considered to adequately sample the lung environment. Compare E to unstained control sample in F. All panels are from the same illustrative sample