Fig. 4

Differential expression of CSGALNACT1 between lung-derived MSCs (LMSCs) from COPD and control. A LMSCs from COPD patients and controls were seeded in duplicates, grown to confluence and serum deprived overnight. Cells were harvested after 24 h and RNA was isolated. mRNA expression of CSGALNACT1 was assessed by qPCR and normalized for housekeeping gene B2M and expressed as 2^−ΔCt. Medians are indicated. Statistical significance was determined using the Mann–Whitney U test. The P values are indicated. P < 0.05 was considered statistically significant. B Decellularized emphysematous tissue lung scaffolds were reseeded with/without COPD or control-derived LMSCs and cultured for 2 weeks. Scaffolds without cells were treated identically to the cell-seeded scaffolds. Paraffin sections were prepared and stained for chondroitin sulfates (CS). Representative images are shown