Fig. 2

Differential gene expression in MSCs from different sources. Lung-derived MSCs (LMSCs), bone marrow-derived MSCs (BM-MSCs) and adipose-derived MSCs (AD-MSCs) from COPD patients and controls were seeded in duplicates, grown to confluence and serum deprived overnight. Cells were harvested after 24 h, RNA was isolated and processed for RNA sequencing. Volcano plots demonstrate differentially expressed genes between the cell types (COPD and control groups combined). Blue represents genes significantly lower expressed; red represents genes significantly higher expressed. False discovery rate (FDR) < 0.05 was used as cut off. A LMSCs vs BM-MSCs. B LMSCs vs AD-MSCs. C BM-MSCs vs AD-MSCs. D Heatmap showing gene sets with specific expression in each cell source. Specific gene expression profile of each source after variance stabilizing transformation (Vst). E Comparison of the expression of KGF, FGF10 and HGF between the different subsets. Medians ± interquartile range (IQR) are shown. F mRNA expression of growth factors KGF, FGF10 and HGF was assessed by qPCR and normalized for housekeeping gene B2M and expressed as 2^−ΔCt. Medians are indicated. Statistical significance was determined using the Mann–Whitney U test. P values are as indicated. * = P < 0.05 between the indicated values. P < 0.05 was considered statistically significant