Fig. 4

SREBP1 regulates TGF-β1-induced SCD1 expression and fibroblast activation. HFL1 cells were treated with TGF‑β1 (10 ng/ml) and Fatostatin HBr (5 µM) for 24 h. A–D A western blot analysis of SREBP1, FASN and SCD1 was performed. E–G qPCR was performed to measure the mRNA levels of ACACA, FASN, and SCD1. H The immunofluorescence staining of HFL1 cells with antibodies against SREBP1 was captured by confocal microscopy. Scale bar, 10 μm. I–L A western blot analysis of fibronectin, COL1A1, and α-SMA was performed. The data are representative of three or six independent experiments and are presented as the means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, as determined by one-way ANOVA with the Tukey‒Kramer post hoc test