Fig. 2

SCD1 inhibition ameliorates airway remodeling but not inflammation in an HDM-induced chronic asthma mouse model. A Schematic diagram of the experimental protocol for HDM sensitization and challenge. B AHR was measured by the lung resistance index (n = 4 mice in each group). C–F The percentages of PAS-positive airway epithelial cells and collagen around the airways were quantified (n = 10 mice in each group). G, H Representative IHC images of collagen I expression in lung sections from the different treatment groups. Scale bar, 100 μm. The collagen I-stained areas were quantified (n = 6 mice in each group). I, J Representative images of HE-stained lung tissue sections from the different treatment groups (n = 10 mice in each group). Scale bar, 100 μm. The inflammation score was determined. K Numbers of total cells in BALF (n = 6 mice for each group). L–O Numbers of differential inflammatory cells in BALF (n = 6 mice in each group). P The total serum IgE levels were assessed by ELISA (n = 6 mice in each group). The data are presented as the means ± SEMs and were statistically analyzed by one-way ANOVA with the Tukey‒Kramer posttest. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001