Fig. 6

The inhibition of p38 MAPK activity attenuated neutrophil recruitment and fibrosis in VG-induced lung injury. Western blot and immunofluorescence analyses confirmed histology and IHC findings, including p38 inhibitor effects on LPS and VG induced neutrophil migration and fibrogenesis. A Western blot analyses showing that intraperitoneal administration of a p38 MAPK inhibitor attenuated the effects of intratracheal administration of LPS or VG on the expression of VLA-4 and VCAM-1 (adhesion molecules) as well as on the expression of the central mediator of fibrogenesis TGF-β and collagen-1 formation. B Immunofluorescence analyses showing that the p38 MAPK inhibitor inhibited LPS- and VG-induced increases in neutrophil recruitment (Ly6G and MPO). *p < 0.05 vs. PBS, ^#p < 0.05 vs. respective group without the inhibitor; N = 6 per group