Fig. 5
From: Gene network analysis for identification of microRNA biomarkers for asthma
miRNA signature profiles in the house dust mite (HDM) mouse model of allergic inflammation. A Principal component analysis (PCI) of lung samples, B PCI of serum samples, red dots represent mice exposed to HDM, and black dots are control mice. C, D Volcano plots of differentially perturbed miRNAs in the lung tissue (C) and serum samples (D), red dots indicate the significantly dysregulated miRNAs (FDR < 0.05), dotted line show the significance threshold (− log10 (FDR) = 1.3). A − log10 (FDR) > 1.3 is considered as statistically significant. E Venn diagram of significantly up-regulated miRNAs in lung (green circle), serum (orange circle) samples, and down-regulated miRNAs in lung (blue circle). The miRNAs included in this analysis have a log2 fold change expression cut off >  + 0.58 and cut off < − 0.58 in HDM vs saline mice. n = 14–16 per group, and FDR < 0.05 is considered statistically significant. The Venn diagram was designed using the online Venny 2.1.0, https://bioinfogp.cnb.csic.es/tools/venny/index.html, with slight modifications. F Heat map of the 213 miRNAs that were substantially dysregulated in the HDM mice vs. saline controls. Red squares show upregulated miRNAs and green represent downregulated miRNAs. The heat map was generated by scaling the data using Z-scores of each miRNA (normalized CPM were converted into z-scores)