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Fig. 5 | Respiratory Research

Fig. 5

From: O-linked N-acetylglucosamine affects mitochondrial homeostasis by regulating Parkin-dependent mitophagy in hyperoxia-injured alveolar type II cells injury

Fig. 5

Effect of O-GlcNAc on apoptosis of RLE-6TN cells induced by hyperoxia. a Effect of O-GlcNAc transferase inhibitors on RLE-6TN cell apoptosis after hyperoxia exposure, as estimated via PI-FITC staining and flow cytometry. b Apoptosis rate of cells treated with different inhibitors of O-GlcNAc transferase after hyperoxia exposure. c Cell cycle distribution as measured via PI staining and flow cytometry. The values are expressed as the ratio of cells in the G0-G1, S, and G2-M phases after treatment with Thiamet G, OSMI-1 and UDP-GlcNAc. d The proportion of cells in each stage of the cell cycle in different groups after hyperoxia exposure (Data are shown as the mean ± SEM (n = 6) and were analyzed using two-way ANOVA followed by Tukey’s post-hoc tests. *P < 0.05, **P < 0.01. NO: normal air-exposed group; HO: hyperoxia-exposed group. HO + TG: 48 h hyperoxia-exposed and Thiamet G-treated group; HO + OS: 48 h hyperoxia-exposed and OSMI-1-treated group; HO + UDP: 48 h hyperoxia-exposed and UDP-GlcNAc-treated group

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Respiratory Research

ISSN: 1465-993X

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