Fig. 4From: O-linked N-acetylglucosamine affects mitochondrial homeostasis by regulating Parkin-dependent mitophagy in hyperoxia-injured alveolar type II cells injuryEffect of O-GlcNAc on the apoptosis of RLE-6TN cells induced by hyperoxia. a Western blot analysis of Parkin and LC3B expression in RLE-6TN lung epithelial cells after exposure to hyperoxia. b Quantification of Parkin and LC3B levels based on (a). c Western blot analysis of Bcl2, Bax, caspase 3 and caspase 9 expression in RLE-6TN lung epithelial cells after exposure to hyperoxia. d The Bcl2/Bax expression ratio based on (c). e Quantification of Bcl2, Bax, caspase 3 and caspase 9 levels based on (c). Data are shown as the mean ± SEM (n = 6) and were analyzed using two-way ANOVA followed by Tukey’s post-hoc tests. *P < 0.05, **P < 0.01. NO: normal air-exposed group; HO: hyperoxia-exposed group. NC: negative control group; TG: Thiamet G-treated group; OS: OSMI-1-treated group; UDP: UDP-GlcNAc-treated groupBack to article page