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Fig. 1 | Respiratory Research

Fig. 1

From: O-linked N-acetylglucosamine affects mitochondrial homeostasis by regulating Parkin-dependent mitophagy in hyperoxia-injured alveolar type II cells injury

Fig. 1

The expression of O-GlcNAc and its metabolic enzymes were downregulated in hyperoxia-exposed RLE-6TN cells. a Western blot analysis of O-GlcNAc, OGA, and OGT levels in hyperoxia-exposed RLE-6TN cells. b–d Gray value analysis of the western blot of O-GlcNAc, OGA, and OGT expression based on a. e Immunofluorescence detection of O-GlcNAc expression in RLE-6TN cells after hyperoxia (magnification × 400; scale bar = 50 µm). f Semi-quantitative analysis of immunohistochemistry based on immunofluorescence. Data are shown as the mean ± SEM (n = 3) and were analyzed using two-way ANOVA followed by Tukey’s post-hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001 compared to the negative control (dimethyl sulfoxide; DMSO) group; NO: normal air-exposed group; HO: hyperoxia-exposed group. NC: negative control group; TG: Thiamet G-treated group; OS: OSMI-1-treated group; UDP: UDP-GlcNAc-treated group

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Respiratory Research

ISSN: 1465-993X

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