Skip to main content

Table 1 Diversity of the cellular models used in e-cigarette toxicological studies (not exhaustive)

From: Considerations on dosimetry for in vitro assessment of e-cigarette toxicity

Cell line

Anatomical location

Cell type

Biological endpoints

References

MSK-Leuk1

Oral

Cancerous from dysplastic leukoplakia near the tongue

Cell viability

Oxidative stress

Genotoxicity

[22]

EpiOral™

Oral

Commercially available primary oral model from MatTek

Histology

Inflammatory mediators

Transcriptomic

[23]

Human primary nasal epithelial cells

Nasal

Primary cells from never-smoker patient

Bacterial adhesion

[24]

Human primary airway epithelial nasal cells

Nasal

Commercially available primary cells from Epithelix

Cell viability

Inflammatory cytokines

Mucins expression

[25]

Oropharyngeal mucosa

Pharynx

Primary cells from healthy patients

Cell viability

Genotoxicity

[26]

Calu-3

Bronchial

Cancerous from lung adenocarcinoma

Inflammatory cytokines

Bacterial adhesion

[27]

NCI-H292

Bronchial

Cancerous from pulmonary mucoepidermoid carcinoma

Oxidative stress

Inflammatory cytokines

Tight junction integrity

[28]

16HBE14o-

Bronchial

SV40-immortalized normal bronchial epithelial cells

Cell viability

Tight junction integrity

[29]

BEAS-2B

Bronchial

Normal bronchial epithelial cells

Oxidative stress

Inflammatory cytokines

Transcriptomic analysis

[30]

CL-1548

Bronchial

hTERT-immortalized primary bronchial epithelial cells

Cell viability

Oxidative stress

[31]

NHBE

Bronchial

Primary bronchial epithelial cells from healthy donors

Cell viability

Oxidative stress

Apoptosis

[32]

MucilAir™

Bronchial

Commercially available primary bronchial model from Epithelix

Tight junction integrity

Ciliary beat frequency

Permeability

[33]

EpiAirway™

Bronchial

Commercially available primary bronchial model from MatTek

Cell viability

Tight junction integrity

[34]

SmallAir™

Bronchial—small airway

Commercially available primary small airway model from Epithelix

Histology

Inflammatory mediators

Transcriptomic analysis

Ciliary beat frequency

[23]

A549

Alveolar

Cancerous from lung adenocarcinoma

Cell viability

Apoptosis

Genotoxicity

[35]

Alveolar Macrophages

Immune system

Primary cells from healthy patients

Phagocytosis

Apoptosis/Necrosis

Inflammatory cytokines

Oxidative stress

[36]

U937

Immune system

Cancerous monocytes from pleural effusion

Cell viability

Oxidative stress

Inflammatory cytokines

[37]