From: Considerations on dosimetry for in vitro assessment of e-cigarette toxicity
Cell line | Anatomical location | Cell type | Biological endpoints | References |
---|---|---|---|---|
MSK-Leuk1 | Oral | Cancerous from dysplastic leukoplakia near the tongue | Cell viability Oxidative stress Genotoxicity | [22] |
EpiOral™ | Oral | Commercially available primary oral model from MatTek | Histology Inflammatory mediators Transcriptomic | [23] |
Human primary nasal epithelial cells | Nasal | Primary cells from never-smoker patient | Bacterial adhesion | [24] |
Human primary airway epithelial nasal cells | Nasal | Commercially available primary cells from Epithelix | Cell viability Inflammatory cytokines Mucins expression | [25] |
Oropharyngeal mucosa | Pharynx | Primary cells from healthy patients | Cell viability Genotoxicity | [26] |
Calu-3 | Bronchial | Cancerous from lung adenocarcinoma | Inflammatory cytokines Bacterial adhesion | [27] |
NCI-H292 | Bronchial | Cancerous from pulmonary mucoepidermoid carcinoma | Oxidative stress Inflammatory cytokines Tight junction integrity | [28] |
16HBE14o- | Bronchial | SV40-immortalized normal bronchial epithelial cells | Cell viability Tight junction integrity | [29] |
BEAS-2B | Bronchial | Normal bronchial epithelial cells | Oxidative stress Inflammatory cytokines Transcriptomic analysis | [30] |
CL-1548 | Bronchial | hTERT-immortalized primary bronchial epithelial cells | Cell viability Oxidative stress | [31] |
NHBE | Bronchial | Primary bronchial epithelial cells from healthy donors | Cell viability Oxidative stress Apoptosis | [32] |
MucilAir™ | Bronchial | Commercially available primary bronchial model from Epithelix | Tight junction integrity Ciliary beat frequency Permeability | [33] |
EpiAirway™ | Bronchial | Commercially available primary bronchial model from MatTek | Cell viability Tight junction integrity | [34] |
SmallAir™ | Bronchial—small airway | Commercially available primary small airway model from Epithelix | Histology Inflammatory mediators Transcriptomic analysis Ciliary beat frequency | [23] |
A549 | Alveolar | Cancerous from lung adenocarcinoma | Cell viability Apoptosis Genotoxicity | [35] |
Alveolar Macrophages | Immune system | Primary cells from healthy patients | Phagocytosis Apoptosis/Necrosis Inflammatory cytokines Oxidative stress | [36] |
U937 | Immune system | Cancerous monocytes from pleural effusion | Cell viability Oxidative stress Inflammatory cytokines | [37] |