Fig. 3

Epidermal growth factor receptor (EGFR) mediates amphiregulin (AREG)-induced cellular communication network factor 2 (CCN2) and fibronectin expressions in A549 human epithelial cells. Cells were transfected with 50 nM of control siRNA (Con siRNA) and EGFR siRNA. After 24 h, cells were stimulated with AREG (10 ng/ml) for the indicated time. Cell lysates were detected using Western blotting and immunodetected with specific antibodies for (A) levels of CCN2, α-tubulin, (B) fibronectin, α-tubulin, (C) phospho-c-Jun N-terminal kinase (JNK) (Thr183/Tyr185), JNK, (D) phospho-c-Jun (serine 63), and c-Jun. Results are expressed as the mean ± SEM, *p < 0.05, n = 3, relative to the AREG-stimulated with the control siRNA group