Fig. 4

The interaction between circGSAP and miR-27a-3p in PMECs. A qRT–PCR was performed to detect miRNA expression levels associated with circGSAP (n = 3). B TargetScan predicted the binding sites of circGSAP and miR-27a-3p, and dual-luciferase assays were used to validate the interactions between circGSAP and miR-27a-3p (n = 3). C Expression levels of miR-27a-3p in lung tissues of IPAH patients were determined by qRT–PCR (n = 4). D Expression levels of miR-27a-3p PMECs were determined by qRT–PCR (n = 6). E Expression levels of miR-27a-3p in lung tissues of control, MCT, MCT-AAV-NC and MCT-AAV group rats (n = 11). F FISH was performed to observe the colocalization of circGSAP and miR-27a-3p in the cytoplasm of PMECs. All data are presented as the mean ± SEM. Scale bar: 100 μm