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Fig. 1 | Respiratory Research

Fig. 1

From: CircGSAP alleviates pulmonary microvascular endothelial cells dysfunction in pulmonary hypertension via regulating miR-27a-3p/BMPR2 axis

Fig. 1

The characteristics, expression and impact of plasma circGSAP on IPAH. A Genomic loci of the circGSAP gene. CircGSAP is produced at the GSAP gene (NM_001350896.2) locus containing exons 15–19. The back-splice junction of circGSAP was identified by Sanger sequencing. B PCR analysis of circGSAP and linear GAPDH in cDNA and gDNA. C Validation of circGSAP by RNase R treatment and PCR analysis. D FISH was used to determine the distribution of circGSAP in PMECs. E Expression levels of circGSAP in plasma samples of IPAH, survivors and nonsurvivors were determined by qRT–PCR. F ROC curves of circGSAP in patients with IPAH, survivors and nonsurvivors with IPAH. G Kaplan–Meier survival analysis for mortality stratified by the cutoff values of circGSAP in IPAH. CircGSAP estimate of survival in patients with IPAH. Control n = 50, IPAH n = 41, survivors  n = 12, nonsurvivors n = 29. All data are presented as the mean ± SEM. Scale bar: 20 μm

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