Fig. 8

HNRNPC knockdown suppressed the lung metastasis in vivo. a, b Stable HNRNPC-knockdown cells (H1299) were transfected with luciferase plasmids and then injected into the tail veins of nude mice. The lung metastatic lesion was then assessed after 7, 18, 25 days. The results showed that HNRNPC knockdown effectively decreased the average radiance of lung metastatic lesion. c, d After 25 days, we used nude mouse lung metastases to make paraffin sections and perform HE staining. Similarly, HNRNPC knockdown caused a reduction of lung metastasis area. e We proposed a model for this study. First, the expression levels of m6A-related genes were correlated with prognosis and immunotherapy response of NSCLC. Next, the m6A regulator HNRNPC was selected as the most influential predictor for NSCLC, and HNRNPC predicted poor prognosis and correlated with tumor invasion and lymph node metastasis. Finally, HNRNPC knockdown inhibited the proliferation and invasion of NSCLC cells and was associated with CD8 + T cell infiltration in TIME. *P < 0.05