Fig. 7

HNRNPC knockdown inhibited the tumor growth in vivo and was associated with CD8 + T cell infiltration in TIME. a In xenograft-bearing mouse models, mouse lung cancer cells (LLC) of stable HNRNPC-knockdown were subcutaneously injected into the left flank of nude mice. The expression of HNRNPC was inhibited after HNRNPC knockdown and the representative figure of tumors formed in each group was exhibited. b, c The tumor weight and tumor volumes were inhibited after HNRNPC knockdown. d In tumor xenografts, the fluorescence density of CD8 was significantly increased after HNRNPC knockdown. Moreover, the fluorescence densities of CD4, CD31 and collagen I were more than that of the control group. e The results of flow cytometry showed that the number of tumor-infiltrating CD8 positive T cells was significantly higher than that of the control group, along with elevated tumor-infiltrating CD4 positive T cells. f IHC staining of CD4 and CD8 was performed in tumor xenografts. Similarly, HNRNPC knockdown caused an obvious increase of tumor-infiltrating CD8 positive T cells, along with elevated CD4 positive T cells. *P < 0.05, **P < 0.01